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Home > Our Company > News and Events

News and Events

Featured Article

Standardized Cryopreservation of Human Primary Cells

Thomas V. Ramos, Aby J. Mathew, Maria L. Thompson, and Rolf O. Ehrhardt3

HemaCare Corporation, Van Nuys, California
BioLife Solutions, Bothell, Washington
BioCision, Larkspur, California

Cryopreservation is the use of low temperatures to preserve structurally intact living cells. The cells that survive the thermodynamic journey from the 37°C incubator to the −196°C liquid nitrogen storage tank are free from the influences of time. Thus, cryopreservation is a critical component of cell culture and cell manufacturing protocols. Successful cryopreservation of human cells requires that the cells be derived from patient samples that are collected in a standardized manner, and carefully handled from blood draw through cell isolation. Furthermore, proper equipment must be in place to ensure consistency, reproducibility, and sterility. In addition, the correct choice and amount of cryoprotectant agent must be added at the correct temperature, and a controlled rate of freezing (most commonly 1°C/min) must be applied prior to a standardized method of cryogenic storage. This appendix describes how human primary cells can be frozen for long-term storage and thawed for growth in a tissue culture vessel.

     More Articles & Publications

Human esophageal myofibroblasts secrete pro-inflammatory cytokines in response to acid and toll-like receptor-4 ligands
Matthew Gargus , Chao Niu , John Vallone , Jana Binkley , Deborah C. Rubin , Anisa Shaker
American Journal of Physiology. 2015 April 16. DOI:10.1152/ajpgi.00333.2014

This article is summarized on HemaCare's blog

 

STING agonist formulated cancer vaccines can cure established tumors resistant to PD-1
Juan Fu, David B. Kanne, Meredith Leong, Laura Hix Glickman, Sarah M. McWhirter, Edward Lemmens, Ken Mechette, Justin J. Leong, Peter Lauer, Weiqun Liu, Kelsey E. Sivick, Qi Zeng, Kevin C. Soares, Lei Zheng, Daniel A. Portnoy, Joshua J. Woodward, Drew M. Pardoll, Thomas W. Dubensky Jr., and Young Kim

Science Translational Medicine; Volume 7, Issue 283, Pages 283ra52, 2015 April 15. DOI: 10.1126/scitranslmed.aaa4306

This article is summarized on HemaCare's blog

 

A Novel Technology for Multiplex Gene Expression Analysis Directly from Whole Blood Samples Stabilized at Ambient Temperature Using an RNA-Stabilizing Buffer
Chang Hee Kim, Majid Abedi, Yenbou Liu, Sree Panuganti, Francisco Flores, Kevin R. Shah, Hannah Catterall, Krishna S. Morampudi, Robert Terbrueggen
Journal of Molecular Diagnostics; Volume 17, Issue 2, Pages 118–127, March 2015

This article is summarized on HemaCare's blog

 

Precision Cancer Immunotherapy: Optimizing Dendritic Cell-based Strategies to Induce Tumor Antigen-specific T-cell Responses Against Individual Patient Tumors.
Osada, Takuya; Nagaoka, Koji; Takahara, Masashi; Yang, Xiao Yi; Liu, Cong-Xiao; Guo, Hongtao; Roy Choudhury, Kingshuk; Hobeika, Amy; Hartman, Zachary; Morse, Michael A.; Lyerly, H. Kim
Journal of Immunotherapy; Volume 38, Issue 4, Pages 155–164, May 2015. DOI: 10.1097/CJI.0000000000000075

This article is summarized on HemaCare's blog

 

Identification of novel human leukocyte antigen-A*0201-restricted, cytotoxic T lymphocyte epitopes on CD133 for cancer stem cell immunotherapy.
Jianfei Ji, Valeria A. Judkowski, Gentao Liu, Hongqiang Wang, Alcinette Bunying, Zhenhua Li, Minlin Xu, James Bender, Clemencia Pinilla and John S. Yu
Stem Cells Translational Medicine. 2014 Mar; 3(3):356-64. DOI: 10.5966/sctm.2013-0135

This article is summarized on HemaCare's blog

 

Optimized Library Prep for Cell-Free DNA from Human Plasma
Jan Risinger, Josh Kinman, Marianna Goldrick

http://www.biooscientific.com

 

The Effect of Glatiramer Acetate Therapy on Functional Properties of B Cells From Patients With Relapsing-Remitting Multiple Sclerosis
Sara J. Ireland, BA; Alyssa A. Guzman, BS; Dina E. O’Brien, BS; Samuel Hughes; Benjamin Greenberg, MD; Angela Flores, MD; Donna Graves, MD; Gina Remington, BSN, RN; Elliot M. Frohman, MD, PhD; Laurie S. Davis, PhD; Nancy L. Monson, PhD
JAMA Neurology. 2014 September 29

 

Cellular Raw Material Collection in Cell Therapy: Critical Determinant of Product Quality
Scott R. Burger, MD, Louis Juliano, and Wenshi Wang, PhD
Drug Discovery World. 2014; Volume I5, Issue 3 - Summer 20I4:29-34.

 

Evaluation of a New Recombinant Antigen-Based Virotech Treponema Pallidum Screen ELISA for Diagnosis of Syphilis
Clemens Busse, Mojdeh Heidary Navid, Andreas Strubel, Paul Schnitzler
Clinical Laboratory. 2013;59(5-6):523-9.

This article is summarized on HemaCare's blog

 

Reduction of total IgE by targeted coengagement of IgE B-cell receptor and FcγRIIb with Fc-engineered antibody
Seung Y. Chu, PhD,* Holly M. Horton, PhD,* Erik Pong, MS, Irene W. L. Leung, PhD, Hsing Chen, MS, Duc-Hanh Nguyen, BS, Cristina Bautista, BS, Umesh S. Muchhal, PhD, Matthew J. Bernett, PhD, Gregory L. Moore, PhD, David E. Szymkowski, PhD, and John R. Desjarlais, PhD
The Journal of Allergy and Clinical Immunology; Volume 129, Issue 4, Pages 1102-1115, April 2012

This article is summarized on HemaCare's blog

 

A novel bispecific antibody format enables simultaneous bivalent and monovalent co-engagement of distinct target antigens
Shufeng Liu, Chao Qiu, Ruidong Miao, Jianhua Zhou, Aram Lee, Baoming Liu, Sandra N. Lester, Weihui Fu, Lingyan Zhu, Linxia Zhang, Jianqing Xu, Daping Fan, Kui Li, Mingui Fu, and Tianyi Wang
PNAS 2013 110 (47) 19083-19088; published ahead of print November 4, 2013

This article is summarized on HemaCare's blog

 

MCPIP1 restricts HIV infection and is rapidly degraded in activated CD4+ T cells
Shufeng Liu, Chao Qiu, Ruidong Miao, Jianhua Zhou, Aram Lee, Baoming Liu, Sandra N. Lester, Weihui Fu, Lingyan Zhu, Linxia Zhang, Jianqing Xu, Daping Fan, Kui Li, Mingui Fu, and Tianyi Wang
PNAS 2013 110 (47) 19083-19088; published ahead of print November 4, 2013

This article is summarized on HemaCare's blog

 

Circulating Tumor Cells: Clinically Relevant Molecular Access Based on a Novel CTC Flow Cell
Jessamine P. Winer-Jones*, Behrad Vahidi, Norma Arquilevich, Cong Fang, Samuel Ferguson, Darren Harkins, Cory Hill, Erich Klem, Paul C. Pagano, Chrissy Peasley, Juan Romero, Robert Shartle, Robert C. Vasko, William M. Strauss, Paul W. Dempsey
PLoS ONE 9(1): e86717. doi:10.1371/journal.pone.0086717

This article is summarized on HemaCare's blog

 

Turning T cells on: epigenetically enhanced expression of effector T-cell costimulatory molecules on irradiated human tumor cells
Anita Kumari, Ercan Cacan, Susanna F Greer and Charlie Garnett-Benson*

Journal for ImmunoTherapy of Cancer 2013, 1:17. doi:10.1186/2051-1426-1-17 (2013)

This article is summarized on HemaCare's blog

 

Acid-citrate-dextrose Formula A versus heparin as primary catheter lock solutions for therapeutic apheresis
Melanie Osby, Pat Barton, Chun Nok Lam, and Minh-Ha Tran
Transfusion. DOI: 10.1111/trf.12310 (2013)

 

A Quantitative Polymerase Chain Reaction Test to Enumerate Leukocytes in Allograft Tissue and the Implications for Donor Eligibility Testing
Cari E. Podzemny, Matthew D. Brenton, Scott A. Brubaker, Russell C. Marians
Cells Tissues Organs; Volume 198, Number 3:221-226. DOI: 10.1159/000355138 (2013)

This article is summarized on HemaCare's blog

 

Four-part leukocyte differential count based on sheathless microflow cytometer and fluorescent dye assay
Wendian Shi, Luke Guo, Harvey Kasdan, and Yu-Chong Tai*
Lab Chip, 2013, 13, 1257. DOI: 10.1039/c3lc41059e

This article is summarized on HemaCare's blog

 

Reduction of total IgE by targeted coengagement of IgE B-cell receptor and FcγRIIb with Fc-engineered antibody
Seung Y. Chu, PhD,* Holly M. Horton, PhD,* Erik Pong, MS, Irene W. L. Leung, PhD, Hsing Chen, MS, Duc-Hanh Nguyen, BS, Cristina Bautista, BS, Umesh S. Muchhal, PhD, Matthew J. Bernett, PhD, Gregory L. Moore, PhD, David E. Szymkowski, PhD, and John R. Desjarlais, PhD
The Journal of Allergy and Clinical Immunology; Volume 129, Issue 4, Pages 1102-1115, April 2012

 

A novel bispecific antibody format enables simultaneous bivalent and monovalent co-engagement of distinct target antigens
Gregory L. Moore*, Cristina Bautista, Erik Pong, Duc-Hanh T. Nguyen, Jonathan Jacinto, Araz Eivazi, Umesh S. Muchhal, Sher Karki, Seung Y. Chu, and Greg A. Lazar
mAbs; 3(6): 546–557; November/December 2011. DOI: 10.4161/mabs.3.6.18123

Company News

Cryoport to offer cryogenic logistics solutions to HemaCare

HemaCare Sells Therapeutic Apheresis Assets to Fresenius Medical Care

Live Video Stream of HemaCare Hosted Panel of Experts at ISCT 2014

Hemacare Hosts Panel of Experts in Cell Therapy at ISCT 2014

BioLife Solutions Announces Strategic Partnership with HemaCare

Hemacare Presents Year End and Six Month Results

Blog Posts

 

Events

06.23.2015 HemaCare will exhibiting at the SITC 30th Anniversary Annual Meeting & Associated Programs taking place from November 4 to November 8 at the Gaylord National Hotel & Convention Center in National Harbor, Maryland. Stop by booth #215 to meet the HemaCare team and learn more about our NEW products and services. For more information on the meeting, please visit SITC's official website at www.sitcancer.org/2015.

 

04.24.2015 HemaCare will exhibiting at the 2015 ISCT Annual Meeting taking place from May 27 to May 30 in Las Vegas, Nevada. Stop by booth #506 to meet the HemaCare team and learn more about our NEW products and services. For more information on the meeting, please visit ISCT’s official website at http://www.isct2015.com/.

 

04.23.2014 HemaCare will exhibiting at the 20th ISCT Annual Meeting taking place from April 23-26, 2014 and be hosting a corporate tutorial titled Sourcing, Isolating, Preserving, Shipping, and Tracking Precious Human Biological Products: Global Solutions for Research and Cell Therapy on April 24th in Paris, France. Stop by booth #49 to meet the HemaCare team and learn more about our NEW products and services. For more information on the meeting, please visit ISCT’s official website at www.celltherapysociety.org.

 

02.03.2014 HemaCare will be attending the 2014 American Association for Cancer Research (AACR) Annual Meeting taking place from April 5-9, 2014 in San Diego, California.  Meet up with our Business Development Executives to learn more about our expanded product offerings and how HemaCare can provide primary cells for your research!  Call (877) 397-3087 to make an appointment. For more information on the meeting, please visit AACR’s official website at www.aacr.org.

 

5.10.2013 HemaCare will be exhibiting at the 2013 ASH Annual Meeting and Exposition taking place from December 7-10, 2013, in New Orleans, LA. Stop by booth #3021 to meet the HemaCare team and learn more about our NEW products and services and enter for a chance to win a prize! For more information on the meeting, please visit ASH's official website at www.hematology.org.

 

2.25.2013 HemaCare will be attending and presenting our abstract at the International Society for Stem Cell Research (ISSCR) Annual Meeting taking place June 12 - 15, 2013 in Boston, MA. For more information on the meeting, please visit www.isscr.org.

 

2.25.2013 HemaCare will be exhibiting and presenting our abstract at The American Association of Immunologists (AAI) Annual Meeting taking place from May 3 - 7, 2013, in Honolulu, HI. Stop by booth #1546 to meet the HemaCare team and learn more about our NEW products and services and enter for a chance to win a prize! For more information on the meeting, please visit AAI's official website at www.aai.org.

 

3.2.2012 HemaCare will be attending the International Society for Cellular Therapy (ISCT) Annual Meeting taking place June 5 – 8, 2012 in Seattle, WA and presenting our presentation entitled Sourcing Biologic Materials and Managing Supply Chains – Quality Considerations.  For more information on the meeting, please visit www.celltherapysociety.org.

 

11.23.2011 HemaCare will be presenting and exhibiting at Biopharmaceutical Development and Production Week: Biopharmaceutical Raw Materials throughout the Product Lifecycle Conference taking place from February 27 - 28, 2012 in San Diego, CA. Stop by booth #205 and say hello and learn more about our products and services. For more information on the meeting, please visit www.ibclifesciences.com

 

11.9.2011 HemaCare will be attending the 6th Annual Stem Cell Meeting on the Mesa taking place from November 30 - December 1 in La Jolla, CA. For more information on the meeting, please visit www.stemcellmeetingonthemesa.com.

 

9.16.2011 HemaCare will be attending the 64th Annual AABB Meeting & CTTXPO taking place from October 22 – 25 in San Diego, CA.  For more information on the AABB meeting, please visit www.aabb.org.

 

9.16.2011 HemaCare will be attending the World Stem Cell Summit taking place from October 3 – 5 in Pasadena, CA.  For more information on World Stem Cell Summit, please visit www.worldstemcellsummit.com.

 

9.16.2011 HemaCare will be attending the Therapeutic Apheresis Academy from September 22-24 in Charlottesville, VA.  For more information on the Academy, please visit www.cmetracker.net.

 

7.15.2011 HemaCare will be attending the upcoming American Association of Clinical Chemistry Annual Meeting in Atlanta, GA.  For more information on AACC, please visit www.aacc.org.